Table 4 Antibodies, their clones, epitopes, pretreatments and dilutions used
Clone/type | Epitope (Immunogen) | Pre-treatment | Dilution | Source | |
|---|---|---|---|---|---|
Alpha-synuclein | 42, Mouse IgG1 | Rat aSN15-123 | 80% Formic acid for 1 hour | 1:1000 | Becton-Dickinson |
Alpha-synuclein | Rabbit polyclonal | a.a. 111-131 | 80% Formic acid for 10 mins | 1:2000 | Millipore |
Tau | AT8, mouse IgG1 | Tau phosphorylated at Ser-202/Thr-205 | None | 1:800 | Autogen bioclear |
Tau | Rabbit Polyclonal | C terminal a.a. 243-441 | None | 1:800 | Dako |
Abeta | 4G8, IgG2b | a.a. 17-24 | 80% FA for 1 hour | 1:2000 | Signet |
Abeta | Rabbit polyclonal | Synthetic peptide corresponding to a.a. 1–16 of Abeta peptides 38, 40, and 42 | 80% FA for 1 hour | 1:500 | Synaptic systems |
MRC1 | Rabbit polyclonal | Macrophage mannose receptor 1 precursor recombinant protein epitope signature tag | Microwave in 10 mM citrate buffer pH 6 for 20 mins | 1:1000 | Prestige antibodies |
Iba1 | Rabbit polyclonal | Synthetic peptide corresponding to C-terminus of Iba1 | Microwave in 10 mM citrate buffer pH 6 for 20 mins | 1:400 | Wako |
CD68 | PG-M1, IgG3 | Fixative-resistant epitope on macrophage-restricted form of CD68 | Microwave in 10 mM citrate buffer pH 6 for 20 mins | 1:500 | Dako |
CD163 | 10D6, IgG1 | Prokaryotic recombinant protein corresponding to domains 1–4 of N-terminal region of CD163 | Microwave in 10 mM citrate buffer pH 6 for 20 mins | 1:50 | Novocastra |
Fibrinogen | Rabbit polyclonal | Fibrinogen isolated from human plasma | Microwave in 10 mM citrate buffer pH 6 for 20 mins | 1:5000 | Dako |