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Table 4 Antibodies, their clones, epitopes, pretreatments and dilutions used

From: Phenotypic profile of alternative activation marker CD163 is different in Alzheimer’s and Parkinson’s disease

  Clone/type Epitope (Immunogen) Pre-treatment Dilution Source
Alpha-synuclein 42, Mouse IgG1 Rat aSN15-123 80% Formic acid for 1 hour 1:1000 Becton-Dickinson
Alpha-synuclein Rabbit polyclonal a.a. 111-131 80% Formic acid for 10 mins 1:2000 Millipore
Tau AT8, mouse IgG1 Tau phosphorylated at Ser-202/Thr-205 None 1:800 Autogen bioclear
Tau Rabbit Polyclonal C terminal a.a. 243-441 None 1:800 Dako
Abeta 4G8, IgG2b a.a. 17-24 80% FA for 1 hour 1:2000 Signet
Abeta Rabbit polyclonal Synthetic peptide corresponding to a.a. 1–16 of Abeta peptides 38, 40, and 42 80% FA for 1 hour 1:500 Synaptic systems
MRC1 Rabbit polyclonal Macrophage mannose receptor 1 precursor recombinant protein epitope signature tag Microwave in 10 mM citrate buffer pH 6 for 20 mins 1:1000 Prestige antibodies
Iba1 Rabbit polyclonal Synthetic peptide corresponding to C-terminus of Iba1 Microwave in 10 mM citrate buffer pH 6 for 20 mins 1:400 Wako
CD68 PG-M1, IgG3 Fixative-resistant epitope on macrophage-restricted form of CD68 Microwave in 10 mM citrate buffer pH 6 for 20 mins 1:500 Dako
CD163 10D6, IgG1 Prokaryotic recombinant protein corresponding to domains 1–4 of N-terminal region of CD163 Microwave in 10 mM citrate buffer pH 6 for 20 mins 1:50 Novocastra
Fibrinogen Rabbit polyclonal Fibrinogen isolated from human plasma Microwave in 10 mM citrate buffer pH 6 for 20 mins 1:5000 Dako