Figure 6

Double immunofluorescence detection of CD163 with Aβ, α-syn and CD68. (a) Double immunofluorescence for CD163 (red) and Aβ (green) in the occipital cortex of an AD case. CD163 immunoreactive microglia clusters around dense cored plaques. (b) Double immunofluorescence for CD163 (red) and α-syn (green) in the frontal cortex of a PD case. CD163 immunoreactive microglia cluster around extracellular LBs. Most of the LBs were intracellular and did not coincide with CD163 immunoreactive microglia. It is predictable that these microglia would react with abnormal aggregates of protein found extracellularly, instead of intracellular pathology. (c) Double immunofluorescence for CD163 (red) and tau (green) in the hippocampus of an AD case. CD163 immunoreactive microglia are in close proximity with neuropil thread but with no specific associations. (d) Double immunofluorescence for CD163 (red) and CD68 (green) in the frontal cortex of an AD case. CD68 is a marker for lysosomes; association (arrow) indicates possible phagocytic properties of CD163 immunoreactive microglia, reinforcing its role as a scavenger receptor.