Evidence of enhanced neuritic and perinuclear LAMP-1 levels. Sagittal brain sections from male 20- to 24-month-old α-Gal A +/0 (a-d) or -/0 (e-h) mice were immunolabeled with an antibody against LAMP-1 and nuclei were counterstained with hematoxylin. Higher magnification areas of interest from α-Gal A -/0 sections are included for detail (i-l). Arrowheads indicate areas of increased perinuclear (f, j) and neuritic (e, g, h, I, k, l) LAMP-1 immunoreactivity, while arrows indicate vascular LAMP-1 staining. The images shown were taken from the cerebellum, pons, hippocampus, and cortex as indicated. Scale bars = 50 microns.