LC3 levels are enhanced throughout α-Gal A-deficient mouse brain. Sagittal brain sections from male 20- to 24-month-old α-Gal A +/0 (a-c, h-j, o-q, u-w) or -/0 (d-f, k-m, r-t, x-z) mice were immunolabeled with an antibody against LC3 (a, d, h, k, o, r, u, x) and counterstained with bis-benzimide (b, e, i, l, p, s, v, y). Inset in panel f indicates LC3-positive puncta. The images shown were taken from the cerebellum (CB), pons (PONS), hippocampus (HIP), and cortex (CTX). Scale bars = 50 microns. Graphs in (g and n) show relative mean fluorescence intensity of LC3 signal normalized to averaged α-Gal A +/0 levels (n = 3) (*p < 0.05, Student’s t-test).