Table 2 Primers and TaqMan probes for KIAA1549-BRAF fusion gene variants
Gene fusion | Primer | TaqMan probe |
|---|---|---|
KIAA1549-BRAF (KIAA exon 13 - BRAF exon 11) forward | GGGTCCCCAGTAAGATCCAG | ATCGCCATGCAGCCGATCCCGGCACCT |
KIAA1549-BRAF (KIAA exon 13 - BRAF exon 11) reverse | CTCGAGTCCCGTCTACCAAG | |
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 9) forward | CGTCCACAACTCAGCCTACATC | ACCACAGGTTTGTCTGC |
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 9) reverse | CCTGGAGATTTCTGTAAGGCTTTC | |
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 11) forward | AGCGATGGCACCTACAGGA | CGTCCACAACTCAGCCTACATCGGATGCCCA |
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 11) reverse | TCATCACTCGAGTCCCGTCT | |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 9) forward | CCAGACGGCCAACAATCC | ACCACAGGTTTGTCTGC |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 9) reverse | CCTGGAGATTTCTGTAAGGCTTTC | |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 10) forward | CAGTGGGGGTCCTTCTACAG | AGCCCAGACGGCCAACAATCCCTGCAG |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 10) reverse | CTTCCTTTCTCGCTGAGGTC | |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 11) forward | AGTGGGGGTCCTTCTACAGC | AGCCCAGACGGCCAACAATCCCTGCAG |
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 11) reverse | CATGCCACTTTCCCTTGTAG | |
KIAA1549-BRAF (KIAA exon 17 - BRAF exon 10) forward | GAATGACTCCCCCGACG | ACCACAGGTTTGTCTGCTACCCCCCCTGC |
KIAA1549-BRAF (KIAA exon 17 - BRAF exon 10) reverse | AGGCTTTCACGTTAGTTAGTGAGC | |
KIAA1549-BRAF (KIAA exon 18 - BRAF exon 10) forward | TGCTGCCAGAGGGATCTACTC | ACCACAGGTTTGTCTGC |
KIAA1549-BRAF (KIAA exon 18 - BRAF exon 10) reverse | CCTGGAGATTTCTGTAAGGCTTTC | |
KIAA1549-BRAF (KIAA exon 19 - BRAF exon 9) forward | CCAGGCTGGCCTTCGTAC | ACCACAGGTTTGTCTGC |
KIAA1549-BRAF (KIAA exon 19 - BRAF exon 9) reverse | CCTGGAGATTTCTGTAAGGCTTTC |