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Figure 8 | Acta Neuropathologica Communications

Figure 8

From: Caspase-mediated cleavage of actin and tubulin is a common feature and sensitive marker of axonal degeneration in neural development and injury

Figure 8

Fractin and TubulinΔCsp6 highlight apoptotic bodies and degenerating neurites in areas of ischemic injury in human brain. Adjacent sections were co-labeled with CC3 (green) and SMI32 (red) (a,d,g), or fractin (green) and neurofilament (red) (b,e,h), or TubulinΔCsp6 (cTub) and neurofilament (red) (c,f,i). Areas of injury contained apoptotic neurons and degenerating axons (Row 1 and Row 2). (Row 1) Insets show DAPI-labeled pyknotic nuclei (arrowhead) in apoptotic corpses that are labeled with CC3 (a), fractin (b) and TubulinΔCsp6 (c). (Row 2) Fractin (e) and TubulinΔCsp6 (f) antibodies highlight degenerating axons, whereas CC3 (d) fails to label degenerating axons via conventional staining techniques. (Row 3) Uninjured areas (g-i) have decreased SMI32 staining and no fractin or TubulinΔCsp6-labeled axons. Scale = 25 μm.

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Acta Neuropathologica Communications

ISSN: 2051-5960

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