Figure 1

Demonstration of specificity of caspase cleavage neoepitope antibodies to actin and tubulin. In vitro cleavage of the substrates was performed via incubation of either recombinant active caspase-3 or −6 with permeabilized brain-derived synaptosomes. A western blot was performed using lysate from this preparation. Blot (a) was colabeled with general anti-alpha-tubulin (tubulin, red) and TubulinΔCsp6 (Cleaved-Tub, green). Blot (b) was colabeled with anti-actin (red) and anti-fractin (green). Recombinant cleaved-caspase-3 (rCC3) generates both cleaved tubulin (a) and fractin (b), whereas recombinant cleaved-caspase-6 (rCC6) only generates only cleaved tubulin (a).