Ultrastructural vessel changes. Normal capillary morphology with surrounding astrocyte end-feet (arrow heads), neuronal projections, identified by synapses (white arrows), and myelinated axons (black arrows) of Gcdh−/+ (a) and Gcdh
(b) mouse cortex after 72 and 12-hours of protein diet exposure, respectively (see insets for enlargement of indicated sections). c Compressed capillary lumen surrounded by edematous myelinated axons (thick black arrows) and dendrites (white arrows) with swelling mitochondria (thin black arrows) of Gcdh
−/− mouse 24-hours after initiation of protein diet. Note intact astrocyte process with nearly normal morphology (*) touching basal lamina of capillary lumen (arrowhead). d Multiple edematous dendrites, identified by synapses (white arrows) and synaptic vesicles expand in proximity to capillary lumen with adjacent astrocyte end-feet (arrowheads). e Compressed capillary surrounded by swollen astrocyte end-feet (black arrowheads) and edematous neuronal projections identified by synapses (white arrows) both with swollen mitochondria. f Severely compromised capillary lumen in Gcdh
−/− mouse cortex at 48-hours of protein diet exposure surrounded by edematous dendrites identified by synapses (white arrows) and multiple swollen mitochondria (thin black arrows) with compressed astrocyte end-feet (black arrowheads). (Perfusion fixed for EM, scale bar = 1 um).