Immunoblot analysis of SphK1, SPL, S1P
and IGF-1R. Tissue lysates from frontal cortex (a) and temporal cortex in the hippocampal area (b) of AD and control brains were assayed for SphK1, SPL, S1P1 and IGF-1R expression. Equal loading of protein was monitored using antibody to β-actin. The intensity of each band was quantified using ImageJ software and is provided as representative under corresponding blot. Values are expressed at 1/1000.