Figure 2

CD4 positive T-cells increase with EAE progression and early tolerisation significantly reduces them. Representative maximum projected confocal images of CD4 positive cells (shown in white) and cell nuclei (blue) in the dorsal funiculus, with zoomed in areas denoted by the white dotted-line boxes from normal (a and g N = 3), day 29 (b and h N = 4), day 105-crEAE (c and i N = 3) and following early (d and j N = 4) and late (e and k N = 5) tolerisation, Scale bars = 100 μm for images a-e and 50 μm for images g-k. These images and the quantification (f) show a significant, progressive increase in CD4 positive cells penetrating the CNS (black asterisk, P < 0.01, one-way ANOVA), that early tolerisation (red bar, P < 0.01, one-way ANOVA) significantly reduces compared to day 105-crEAE back to a similar number as at day-29. Late tolerisation (green bar, f) had no significant effect compared to day 105-crEAE.