Negative heavy metal staining in different CNS regions (all from the same MND patient in Figure
B, C and D). No heavy metal staining was present in: (A) Neuromelanin-containing neurons of the substantia nigra. Bar = 25 μm. (B) Neurons in the dentate gyrus of the hippocampus. Bar = 50 μm. (C) Purkinje cells (arrow) in the cerebellar cortex. Bar = 20 μm. (D) Lipofuscin-containing neurons in the cerebellar dentate nucleus. Bar = 25 μm. and (E) Lipofuscin-containing neurons in the inferior olivary nucleus. Bar = 30 μm. AMG and hematoxylin.