Analysis of terminal diseased EAM / control mice. a) Kaplan-Meier curve showing no differences in survival probability in prion inoculated EAM-mice when compared to controls. b) Western blots of muscle, brain and spleen of terminal sick EAM / control mice. PrPSc loads are variable without significant differences between groups. As positive control, brain homogenate from a terminally diseased wild type mouse is loaded with and without PK-digestion. As negative control brain homogenate from a healthy wild type mouse is loaded with PK-digestion (molecular weights indicated in kDa). c) H&E as well as immunohistochemical staining for T-cell marker CD3 show signs of myositis in the muscle tissue only in EAM mice but not controls. Nevertheless, in brain tissue in both cohorts a pronounced gliosis could be detected by H&E and immunohistochemical staining for astrocyte marker GFAP (Scale bar is 100 μm and 20 μm for muscle and 200 μm and 20 μm for brain).