Figure 1
From: Myositis facilitates preclinical accumulation of pathological prion protein in muscle
Analysis of muscle tissue derived from myositis-induced SJL/J mice and untreated controls. a) H&E- as well as Elastica-von-Giesson staining show a high number of infiltrating immune-cells at day 21 after the immunization with myosin, which is followed by a milder invasion of inflammatory cells and necrosis of single muscle fiber at day 54. Immunohistochemical stainings of B-, T-cells and FDCs reveal that most of the infiltrating cells are T-cells and that only very few B- cells or FDCs can be detected. (Scale bar is 50 μm). b) Western blot analysis of PrPC of muscle, brain and spleen. Beta-actin (for muscle and brain) and coomassie-staining (for spleen) are shown as loading controls (molecular weights indicated in kDa). c) Quantification of PrPC expression in muscle, brain and spleen. Values were adjusted to loading controls. Note that there is a peak of PrPC expression at day 60 in muscle as well as in spleen in myositis-induced mice.