Figure 5

Brain reperfusion, or glucose administration re-established ABCB1 protein levels in hypoperfused brain capillaries. Western blot analysis using brain capillary extracts shows that 24 after brain reperfusion, or glucose administration in 24 hours hypoperfused mice, (A) tyrosine phosphorylation (pY) of GSK3β is decreased, (B) β-catenin nuclear abundance is rescued, and (C) ABCB1 protein returned to normal levels in the ipsilateral hemisphere. (D) 1% Thioflavin S staining is not detected in the hypoperfused brain capillaries 24 hours after brain reperfusion, or glucose administration in 24 hours hypoperfused mice. Data are means ± SEM (n = 4). C, contralateral non-hypoperfused brain capillaries; I, ipsilateral hypoperfused brain capillaries; 1 VO, one vessel occlusion for 24 hours; S, saline; R, brain reperfusion; G, glucose administration. **P < 0.01 / ****P < 0.0001 compared with contralateral saline (One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test).