Peripherally applied human Aβ
peptides form small aggregates in the hypoperfused hemisphere. Stacked images obtained from two-photon intravital imaging examining brain distribution and deposition of peripherally applied human Aβ1-42 Hilyte Fluor 555 (25 ng), 3 hours (A), and 6 weeks (B) after intravenous injection of 24 hours hypoperfused mice. (A) Aβ1-42 Hilyte Fluor 555 is detected mostly around ipsilateral brain capillaries 3 hours after injection. (B) Congo Red positive Aβ aggregates are exclusively detected in the hypoperfused hemisphere. Two video sections were generated from these stacks (Additional file 3: Video S1 = Ipsiateral hemisphere; Additional file 4: Video S2 = Contralateral hemisphere). The same mouse was used during the time course (n = 2). 1 VO 24 H, one vessel occlusion for 24 hours.