Figure 3
Peripherally applied human Aβ 1-42 peptides enter the hypoperfused hemisphere. Stacked images obtained from two-photon intravital imaging examining brain distribution of peripherally applied human Aβ1-42 Hilyte Fluor 555 (10 μg), 3 hours, and 48 hours after intravenous injection of 24 hours hypoperfused mice. Aβ1-42 Hilyte Fluor 555 is detected in large quantities in the ipsilateral hemisphere around brain capillaries after injection, but the signal was barely detectable in the contralateral hemisphere at 3 and 48 hours. The same mouse was used during the time course (n = 2). 1 VO 24 H, one vessel occlusion for 24 hours.