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Figure 4 | Acta Neuropathologica Communications

Figure 4

From: In vivo modification of Abeta plaque toxicity as a novel neuroprotective lithium-mediated therapy for Alzheimer’s disease pathology

Figure 4

Lithium treatment reduced the size and increased the compaction of the extracellular Abeta plaques. A) Lithium treatment reduced the size of Abeta deposits. Plaques were immunostained with anti-Abeta42 antibody. Representative images (a1 and a2) and quantitative analysis (a3 and a4) of Abeta plaque size from the CA1 subfield of control and lithium-treated PS1xAPP mice. (a3) The individual plaque size (μm2) was quantitatively assessed from 25 sections of 6 different control or lithium treated PS1xAPP mice. (a4) The plaque size distribution was determined by calculating the number of plaques falling into distinct area categories (ranging from <200 μm2 to >2000 μm2). For each category, the difference between control and lithium was determined by t-test. so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. B) Lithium treatment increased the Abeta plaque compaction. Signal density of Abeta42 immunostained plaques from control (b1, b2) and lithium-treated (b3, b4) mice were measured in the CA1 subfield of hippocampus. Optical densities (pixel/μm2) of plaques from 5 sections/mouse and 5 mice per group was represented in the graph. C) Plaque oligomeric halo, considered as the difference between OC- and Thio-S-stained areas was reduced by lithium. Plaques were sequentially staining with Thio-S and the OC antibody. (c1-c6) Representative images of a similar size plaque in the CA1 region of control (c1-c3) or lithium-treated (c4-c6) mice showing the plaque halo. (c7) Similar size plaques (< 200 μm2) were analyzed (15 plaques per animal, n = 3 mice per group). Scale bars: a1, a2 200 μm; b1-b4 25 μm; c1-c6 20 μm.

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