Figure 8

CDI analysis of cases compared with negative control groups. (a) Comparison of CDI values (means) for non-CJD control, sudden death control and cerebral cortex samples. Frontal cortex samples from ten cases from the Medical Research Council Edinburgh Brain and Tissue bank were analysed by CDI as controls. Five of these were Sudden Death cases with no history of a neurological condition (‘Sudden death’), whereas another five were cases initially referred to NCJDRSU as suspected CJD cases, but given an alternative final diagnosis (‘non-CJD’). These samples were analysed in comparison with three samples of cerebral cortex from cases 1 (one sample) and 2 (two samples). The upper and lower dashed lines indicate the D-N cut-off values, these being the [mean + (2.5 X Standard Deviation)] for sudden death and non-CJD cerebral cortex tissue, respectively. (b) Comparison of CDI values (means) for non-CJD control and sudden death control versus the individual samples from cases 1 and 2. (c) The D-N values are shown from CDI analysis of a sample of frontal cortex from cases 1 and 2 (striped bar) and sudden death cases (black bar) or non-CJD neurological control cases (grey bars), following treatment with various concentrations of PK as indicated on the x-axis.