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Figure 6 | Acta Neuropathologica Communications

Figure 6

From: Astrocytes differentially respond to inflammatory autoimmune insults and imbalances of neural activity

Figure 6

Upregulation of GFAP and AQP4 in the cerebellum in AnkG and Kv3.1 KO mice. A, High magnification image stacks of cerebellar molecular layer stained with anti-PKCγ (green) and anti-GFAP (red) antibodies. The collapsed 2D image is on the left, and 3 cross sections are on the right. B, Confocal image stacks from the AnkG KO mice. C, Confocal image stacks from the Kv3.1 KO mice. In (A,C), the crossbars reveal radially oriented GFAP + Bergmann glial processes in (A) WT and (C) Kv3.1 KO mice. In (B), the crossbars are centered on highly upregulated GFAP + astrocyte processes in the absence of Purkinje neurons in an AnkG KO mouse. D, A single confocal image of the granule cell layer in a WT mouse. E, An image from the AnkG KO mice. F, An image from the Kv3.1 KO mice. G, Normalized fluorescence intensity in the molecular layer. H, Normalized fluorescence intensity in the granule cell layer. (G, H) One-Way ANOVA followed by Fisher’s test. *, significant difference from Wildtype for each antibody, p < 0.05. **, significant difference from Wildtype for each antibody, p < 0.01. The “n” number of quantified images is provided for each bar. Scale bars, 50 μm.

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