Alteration of GFAP and AQP4 in the cortex of EAE mice. A, Alterations of astrocytic proteins in the cortex at different stages of rrEAE. B, Summary of the alterations of intensities. C, Summary of increased GFAP + cells. (B, C) One-Way ANOVA followed by Fisher’s test. *, significant difference from Control for each antibody, p < 0.05. **, significant difference from Control for each antibody, p < 0.01. The “n” number of quantified images is provided for each bar. High magnification confocal image stacks were obtained from control (D) and EAE (E) Thy1-YFP transgenic mice. Images contain YFP (green), GFAP (blue) and AQP4 (red). The collapsed 2D image is on the left, and 3 cross sections are on the right. Scale bars, 200 μm in A, 50 μm in D and E.