Images of double staining against NG2 (clone 9.2.27)/GFAP and NG2 (clone 9.2.27)/Iba-1. (A) Co-localisation between GFAP+ astrocyte (red) and granular NG2 (green). (B) No co-localisation between iba-1+ microglia (red) and granular NG2 (green). C-E represent images of an NG2 and GFAP staining in a higher magnification, where (C) shows both ramified (arrow) and clustered (arrowhead) NG2, (D) shows a GFAP+ astrocyte and (E) a merged picture of the two stainings. F-G represent images of NG2 and Iba-1 stainings in a higher magnification where (F) shows ramified NG2 cells (arrowhead), (G) Iba-1+ microglia and (H) a merged picture of the two stainings. Scale bar A, B=20 μm and C-H=15 μm.