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Table 3 Antibodies used for immunohistochemistry and for immunoblotting

From: The link between intraneuronal N-truncated amyloid-β peptide and oxidatively modified lipids in idiopathic autism and dup(15q11.2-q13)/autism

Name Epitope or target Dilution Host/type Source
6E10 4-13 aa Aβ 1:2,000 M-monocl Signet Laboratories (antibody developed at IBRDD [30, 31])
4G8 17-21 aa Aβ 1:2,000 M-monocl IBRDD [30, 32]
R226 36-42 1:40 R-polycl. IBRDD [33]
Anti-MDA malondialdehyde 1:500 R-polycl Alpha Diagnostic Int., San Antonio, TX
G-polycl
Anti-HNE 4-hydroxy-2-nonenal 1:500 R-polycl Alpha Diagnostic Int., San Antonio, TX
COX IV Mitochondria 1:100 R-monocl Cell Signaling Technology
LAMP 1 Lysosomes 1:400 R-polycl Abgent
actin actin 1:4000 M-monocl Pierce/Thermo Sci., Rockford, IL
LC3B Autophagic vacuoles 1:100 R-monocl Cell Signaling Technology Danvers, MA
8C4 Tripeptidyl peptidase I 1:30 M-monocl IBRDD [34]
GFAP Astrocytes 1:400 R-polycl Sigma, St. Louis, MO
SP15 synaptophysin 1:100 M-monocl Calbiochem-EMD Biosciences, Inc., La Jolla, CA
synaptophysin synaptophysin 1:200 G-polycl GeneTex, Irvine, CA
  1. Antibodies were monoclonal or polyclonal: Mouse (M); Rabbit (R) or Goat (G).