Table 3 Antibodies used for immunohistochemistry and for immunoblotting
Name | Epitope or target | Dilution | Host/type | Source |
|---|---|---|---|---|
6E10 | 4-13 aa Aβ | 1:2,000 | M-monocl | |
4G8 | 17-21 aa Aβ | 1:2,000 | M-monocl | |
R226 | 36-42 | 1:40 | R-polycl. | IBRDD [33] |
Anti-MDA | malondialdehyde | 1:500 | R-polycl | Alpha Diagnostic Int., San Antonio, TX |
G-polycl | ||||
Anti-HNE | 4-hydroxy-2-nonenal | 1:500 | R-polycl | Alpha Diagnostic Int., San Antonio, TX |
COX IV | Mitochondria | 1:100 | R-monocl | Cell Signaling Technology |
LAMP 1 | Lysosomes | 1:400 | R-polycl | Abgent |
actin | actin | 1:4000 | M-monocl | Pierce/Thermo Sci., Rockford, IL |
LC3B | Autophagic vacuoles | 1:100 | R-monocl | Cell Signaling Technology Danvers, MA |
8C4 | Tripeptidyl peptidase I | 1:30 | M-monocl | IBRDD [34] |
GFAP | Astrocytes | 1:400 | R-polycl | Sigma, St. Louis, MO |
SP15 | synaptophysin | 1:100 | M-monocl | Calbiochem-EMD Biosciences, Inc., La Jolla, CA |
synaptophysin | synaptophysin | 1:200 | G-polycl | GeneTex, Irvine, CA |