Figure 9

Brains of individuals diagnosed with dup(15)/autism, 10 and 11 years old. Confocal microscopy revealed Aβ in more than 50% of frontal cortex neurons in the dup(15) with autism. The immunoreactivity for HNE is granular and is located in neurons, glia and neuropil. Intracellular deposits of Aβ are the sites of strong accumulation of MDA (a), but in one case of dup(15)/autism (b), large pyramidal neurons in layers 3 and 5 in the cortex accumulating Aβ contain a particularly strong immunoreaction for MDA in the vicinity of plasma membrane. The reactions for lipid peroxidation products are correlated with cellular immunoreactivity for Aβ. Bars 10 μm. Measurements of the intensities of immunoreactions for Aβ and HNE in all dup(15) cases listed in Table  1 shown in the graph reveal two populations of neurons with distinct intracellular Aβ/HNE ratios.