HNE- and MDA-modified proteins in brain lysates. (a) HNE- and MDA-modified proteins detected by immunoblotting in frontal cortex lysates of autism and control brains (lanes L). More than 85% of the modified proteins were detected in supernatant (S) after centrifugation at 10,000 g for 10 min, but not in the pellet (P). (b) The levels of total MDA- and HNE-modified proteins in lysates normalized to actin levels, as detected by slot blotting in all brains listed in Table
2, were similar in dup(15)/autism, idiopathic autism and in controls.