Figure 3
Detection of alpha synuclein oligomers in the striatum. The neuronal projections in the striatum of rats injected with AAV-V1S and AAV-SV2 in the SN were imaged for the presence of venusYFP fluorescence. Coronal sections from the striatum of rats injected into the SN were imaged under fluorescence microscope. VenusYFP is directly detected and localized to the striatum in animals injected with V1S and SV2, no fluorescence is detected contralateral (a). VenusYFP fluorescence is also detected in the striatum of rats injected with AAV-venus (b). Punctate fluorescence pattern is detected only in the striatum of animals injected with V1S and SV2 compared to the diffuse staining observed in animals injected with full length venusYFP (upper panel), suggestive for a possible accumulation of alpha synuclein within the striatum. Representative images are displayed. Scale bar 500 ÎĽm.