Figure 1From: Excitotoxin-induced caspase-3 activation and microtubule disintegration in axons is inhibited by taxol Timecourse of axonal and dendritic alterations following somatodendritic exposure to kainic acid. Top panel show schematic of neuronal arrangement in microfluidic chambers. Β-III tubulin (axonal) and MAP2 (dendrite) immunoreactivity indicated beading of a small number of both distal axons and distal dendrites by 1 hour post exposure to kainic acid. By 6 hours extensive axonal beading was present and dendritic arbours were reduced in area. By 16 hours axons were fragmented, although β-III tubulin immunoreactivity was still present within axonal fragments and MAP2 immunoreactivity was restricted to neuronal soma and a small number of short dendrites. Arrows indicate beading; arrow head indicates shortened dendrites. Scale bar: 20 μm.Back to article page