Figure 5

Immunohistochemical staining of cortical sections of 5XFAD transgenic mice. (a) Immunostaining with IC16 demonstrating intraneuronal Aβ accumulation in 6 week old hemizygous 5XFAD. (b) Homozygous 5XFAD mice exhibited more intensive intraneuronal staining at the same age. (c, d) Abundant extracellular plaque staining with IC16 at the age of 12 months. (e) No signal was detected using NT4X-167 in 6 week old hemizygous 5XFAD. (f) In homozygous 5XFAD mice significant intraneuronal staining was observed with NT4X-167. (g, h) Abundant extracellular plaque staining with NT4X-167 at the age of 12 months. (i, j) No intraneuronal Aβ was observed with the pyroglutamate specific antibody 1–57 in 6 week-old hemizygous (i) and homozygous (j) 5XFAD mice. (k, l) Extracellular plaque staining with 1–57 at the age of 12 months. At 12 months only hemizygous 5XFAD are used. Abbreviations: w, week; m, month. Scale bar in k for a-c, e-g and i-k: 50 μm, and in l for d, h and l: 200 μm.