SDS-PAGE Western blot analysis of IC16, 1–57 and NT4X-167 antibodies. Freshly dissolved synthetic Aβ variants (7 μg each) were probed to a membrane. (a) IC16 detects Aβ1–40 and Aβ1–42 monomers and low molecular weight aggregates. (b) 1–57 recognizes AβpE3-40 and AβpE3-42 monomers, low molecular weight and larger aggregates of AβpE3-42. (c) NT4X-167 recognizes monomers and low molecular weight aggregates derived from AβpE3-40, AβpE3-42, Aβ4–40 and Aβ4–42.