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Table 1 List of the primary antibodies used in this study

From: The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer’s disease

Antibody Species Dilution Supplier/cat. number
Anti-β amyloid 1-16 amino acid (6E10) Mouse (monoclonal) 1: 1000 for IHC Covance cat number (SIG 39320)
1:2000 for WB
Anti-Hepcidin Rabbit (polyclonal) 1: 200 for IHC Abcam (Ab30760) (0.9  mg/ml)
1:100 for WB
Anti-Ferroportin (SLC40A1) Mouse (monoclonal) 1: 1000 for IHC Abcam (ab93438) (1 mg/ml)
Anti- Ferroportin (SLC40A1) Rabbit (polyclonal) 1:200 for IHC Abcam (ab85370) (1 mg/ml)
Anti-NG2 Mouse (monoclonal) 1:500 for IHC Abcam (ab83508) (0.5 mg/ml)
Anti-NG2 Rabbit (polyclonal) 1:500 for IHC Abcam (ab83178) (0.4 mg/ml)
Anti-GFAP Mouse (monoclonal) 1:1000 for IHC Sigma (G3893 Clone G-A-5)
Anti-GFAP Rabbit (polyclonal) 1:200 for IHC Abcam (ab48050) (1 mg/ml)
Anti-βIII-tubulin Mouse (monoclonal) 1:1000 for IHC Millipore (clone 2G10, neuronal | 05-559)
Anti β-actin Mouse (monoclonal) 1:10000 for WB Sigma (clone AC-74, A5316 )
Anti-APOE Mouse (monoclonal) 1:1000 for WB Abcam (ab1907) (1 mg/ml)
1:500 for IHC
Anti-APOE Rabbit (polyclonal) 1:100 for WB Abcam (ab85311) (0.1 mg/ml)
1:100 for IHC
Anti-Ferritin heavy chain Rabbit (polyclonal) 1:200 for IHC Abcam (65080) (1 mg/ml)
Anti-Ferritin light chain Rabbit (polyclonal) 1:250 for IHC Abcam (69090) (0.8 mg/ml)
Anti-Ferritin light-chain Mouse (monoclonal) 1: 500 for IHC Abcam (10060) (1 mg/ml)
1:1000 for WB  
Anti-Myelin basic protein Mouse (monoclonal) 1:500 for IHC Abcam (ab62631) (1 mg/ml)
Anti-CD31 (JC/70A) Mouse (monoclonal) 1:100 for IHC Abcam (ab9498) (0.15 mg/ml)
Anti-CD31 Rabbit (monoclonal) 1: 1000 for IHC Abcam (ab32457) (1 mg/ml)
Anti-PDGFβR1 Mouse (monoclonal) 1: 1000 for IHC Abcam (ab32570) (1 mg/ml)
  1. IHC, immunohistochemistry; WB, Western blotting.
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Acta Neuropathologica Communications

ISSN: 2051-5960

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