Table 1 List of the primary antibodies used in this study
Antibody | Species | Dilution | Supplier/cat. number |
|---|---|---|---|
Anti-β amyloid 1-16 amino acid (6E10) | Mouse (monoclonal) | 1: 1000 for IHC | Covance cat number (SIG 39320) |
1:2000 for WB | |||
Anti-Hepcidin | Rabbit (polyclonal) | 1: 200 for IHC | Abcam (Ab30760) (0.9 mg/ml) |
1:100 for WB | |||
Anti-Ferroportin (SLC40A1) | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab93438) (1 mg/ml) |
Anti- Ferroportin (SLC40A1) | Rabbit (polyclonal) | 1:200 for IHC | Abcam (ab85370) (1 mg/ml) |
Anti-NG2 | Mouse (monoclonal) | 1:500 for IHC | Abcam (ab83508) (0.5 mg/ml) |
Anti-NG2 | Rabbit (polyclonal) | 1:500 for IHC | Abcam (ab83178) (0.4 mg/ml) |
Anti-GFAP | Mouse (monoclonal) | 1:1000 for IHC | Sigma (G3893 Clone G-A-5) |
Anti-GFAP | Rabbit (polyclonal) | 1:200 for IHC | Abcam (ab48050) (1 mg/ml) |
Anti-βIII-tubulin | Mouse (monoclonal) | 1:1000 for IHC | Millipore (clone 2G10, neuronal | 05-559) |
Anti β-actin | Mouse (monoclonal) | 1:10000 for WB | Sigma (clone AC-74, A5316 ) |
Anti-APOE | Mouse (monoclonal) | 1:1000 for WB | Abcam (ab1907) (1 mg/ml) |
1:500 for IHC | |||
Anti-APOE | Rabbit (polyclonal) | 1:100 for WB | Abcam (ab85311) (0.1 mg/ml) |
1:100 for IHC | |||
Anti-Ferritin heavy chain | Rabbit (polyclonal) | 1:200 for IHC | Abcam (65080) (1 mg/ml) |
Anti-Ferritin light chain | Rabbit (polyclonal) | 1:250 for IHC | Abcam (69090) (0.8 mg/ml) |
Anti-Ferritin light-chain | Mouse (monoclonal) | 1: 500 for IHC | Abcam (10060) (1 mg/ml) |
1:1000 for WB | |||
Anti-Myelin basic protein | Mouse (monoclonal) | 1:500 for IHC | Abcam (ab62631) (1 mg/ml) |
Anti-CD31 (JC/70A) | Mouse (monoclonal) | 1:100 for IHC | Abcam (ab9498) (0.15 mg/ml) |
Anti-CD31 | Rabbit (monoclonal) | 1: 1000 for IHC | Abcam (ab32457) (1 mg/ml) |
Anti-PDGFβR1 | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab32570) (1 mg/ml) |