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Figure 7 | Acta Neuropathologica Communications

Figure 7

From: The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer’s disease

Figure 7

Ferritin accumulation in the APP-tg mouse brain. DAB immunostaining of 10 month-old APP-tg mice showed intraneuronal and extracellular accumulation of Aβ peptides present throughout the brain, including in the hippocampus, dentate gyrus and all six layers of cortex (a). Another section from cortex stained with ferritin light chain (FTL) showed immunoreactivity throughout cortex, as well as in plaques (b-c) and centre core of the plaques (d). Double-label IFC staining of Aβ42 and FTL, seen close to the blood vessels in the hippocampus and both proteins were detected throughout the brain (e-g). Ferritin heavy chain (FTH) was visible in a selected population of neurons in the hippocampus but did not co-localise with Aβ42 (h-j). Hepcidin was seen in GFAP positive astrocytes (k-m). Protein levels were quantified by Western blotting from 6 month-old APP-tg mice and age matched controls (n = 6). Aβ42 levels were higher in APP-tg mice compared to control (p < 0.0002) while hepcidin levels were decreased (p<0.005). Ferroportin, ApoE and FTL levels, however, did not significantly change (n-o). Scale bar a-b 250 μm, c 100 μm, d 25 μm, e-j 75 μm, k-m 30 μm.

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