Hepcidin protein in mature amyloid plaques in APP-tg mouse brain. Confocal analysis of Aβ plaques and hepcidin co-localisation in brains from 10 month-old APP-tg mice. In the hippocampus (CA1), hepcidin granules were visible in the cytosol of selected neurons (a) and most of the neurons were positive for Aβ42 (b-c). Individual deposits were analysed by confocal laser scanning microscopy and z-stacks were prepared. Hepcidin was present in surviving neurons in the DG, distributed around the periphery of amyloid plaques in a form of a “halo” indicating wagon-wheel plaques (d-f). Arrowheads indicate dual-labeled plaques. Scale bar a-f 25 μm.