DAF treatment reduces expression of aquaporin-4 in the animal brain cortex post BOP exposure. Representative photomicrographs of frozen brain sections were immunostained with anti-aquaporin-4 antibody (top panel) and the total fluorescence of aquaporin-4 (bottom panel) Original magnification of × 200. Scale bar, 200 μm. Group data is expressed as mean ± SEM and compared using one-way ANOVA followed by Bonferroni test. n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.