Deposition of rhDAF in the brain cortex and hippocampus of animals treated with rhDAF. Representative photomicrographs of frontal grey matter ( a ) and hippocampal DG ( b ) from the frozen sections of rat brains immunostained by anti-human DAF (red) and anti-endothelial cell (EC, green) antibodies. Original magnification of × 200. Scale bar, 200 μm. n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.