Blast-induced shear-related injury in the brain. Shown are sections from a rat sacrificed four months after receiving a 3 × 74.5 kPa blast exposure. Diascopic bright/dark field images (A) and H&E stained sections (B-C) are shown that demonstrate a discontinuity in the tissue (indicated by arrows in all panels) in contrast to the normal appearance of the contralateral side in panels (A) and (B). Panel (D) shows a reconstructed image of the area around the lesion immunostained with an anti-collagen IV antibody, which stains mainly blood vessels, and is counterstained with cresyl violet. The external capsule (ec) as well as the CA1 pyramidal cell layer and the dentate gyrus (DG) of the hippocampus are indicated. The discontinuity in the hippocampal pyramidal cell layers is indicated by arrows. Chronic disruption of the perirhinal cortex, external capsule and hippocampus are visible. Scale bars: 1 mm, A-B; 150 μm, C; 100 μm, D.