Figure 2

Pathological findings at the needle tract. The extent of AQP4 loss (a-c) and the infiltration of W3/13⁺ granulocytes (d-f), ED1⁺ macrophages/activated microglial cells (g-i), and CD3⁺ T cells (j-l) are presented. All histological samples and quantitative data (c,f,i,l) shown derive from the striatum of 3-week old Lewis rats injected with 30 ng of IL-1β (histological data), and of IL-1β, TNF-α, IL-6, IFN-γ, CXCL2, or vehicle (PBS, veh) as indicated (quantifications). Animals were seropositive for NMO IgG or control IgG as indicated. Statistically significant differences between individual cytokine treatments are shown (*p<0.05, ANOVA-Dunett T3). Scale bars: 1 mm (a,d,g,j) and 100 μm (b,e,h,k).