Figure 8

Schematic flowchart of the cell death studies. Cell death was identified using the TUNEL assay in HeLa cells. Cells were transfected with GFP and the wild type TDP-43 or mutated TDP-43 plasmid. About 1000 cells of each sample were counted. After treatment with Peptide B and C, the amount of cells with aggregation decreased. However, despite the reduction in aggregation, increased cell death was observed in the non-aggregated cells. This suggests that the peptides can inhibit aggregation but not cell death. In addition, the very low percentage of cell death in untransfected cells suggests the peptides are not toxic to cells.