Quantitative evaluation of neuron densities and layer-specific gene expression in controls and FCD cases. (a,b) Quantification of NeuN-positive neurons in layers III (a) and V (b) of control cases and different types of FCD. NeuN immunolabeled neurons were counted as described in Material and Methods. Note that cell density (number of cells/mm2) did not differ between controls or FCD cases (one-way ANOVA). (c,d) Quantification of RORß (c) or ER81 (d) mRNA expression by real time RT-PCR. (control: n = 4; control + AHS: n = 3; FCD IIIa: n = 13; FCD TCx: n = 3; FCD FCx: n = 4). (c) Expression levels of RORß mRNA were similar in all groups (one-way ANOVA). (d) Expression levels of ER81 mRNA were comparable between controls and FCD cases (one-way ANOVA). TCx, temporal cortex; FCx, frontal cortex.