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Figure 2 | Acta Neuropathologica Communications

Figure 2

From: Establishing quantitative real-time quaking-induced conversion (qRT-QuIC) for highly sensitive detection and quantification of PrPSc in prion-infected tissues

Figure 2

Directly detecting purified PrP27-30 with immunoblotting and ThT-binding fluorescence. (a) Purified mouse RML and ME7 scrapie-prion PrP27-30 was serially diluted and detected by immunoblotting. Aliquots were digested with 100 μg/ml Proteinase K (PK) before loading on the gel. Undigested PrPC is shown as a migration control. Signals were detected by 4H11 monoclonal antibody. Mr is shown on the right. (b) Fluorescence of purified RML and ME7 PrP27-30 and normal PrPC was measured after ThT-binding. The mean and s.e.m. are shown (n = 5).

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