Table 1 Human gene sequences for ApoE, βAPP, α-secretase, BACE 1, BACE 2, IL-1α, IL-1β, and S100B, with PCR annealing temperatures and number of amplification cycles
From: Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype
Gene analyzed | Human sequences | Annealing temp. (Co) | Cycle no. |
|---|---|---|---|
ApoE | F: CCCAGGTCACCCAGGAACT | 60 | 40 |
R: AGTTCCGATTTGTAGGCCTTCA | |||
βAPP | F: AACCACCGTGGAGCTCCTT | 60 | 40 |
R: ATGCCACGGCTGGAGATC | |||
α-secretase (ADAM 10) | F: AGTGTACGTGTGCCAGTTC | 60 | 40 |
R: TTGCAGGGTGATGGTTCG | |||
BACE 1 | F: CAGTCAAATCCATCAAGGCAG | 60 | 40 |
R: GTTGGTAACCTCACCCATTAGG | |||
BACE 2 | F: AGAGTATAACGCAGACAAGGC | 60 | 40 |
R: CCAGTCCAGAAACCATCAGAG | |||
IL-1 α | F: GGAGAGCATGGTGGTAGTAGCAA | 60 | 40 |
R: TGGCTTAAACTCAACCGTCTCTT | |||
IL-1 β | F: GCACGATGCACCTGTACGAT | 60 | 40 |
R: CACCAAGCTTTTTTGCTGTGAGT | |||
S100B | F: CCCCAGGGACTCTTGTTAACAG | 60 | 40 |
R: CACGGTGCACGCTTTATCAC |