Figure 2

APOE genotype influences expression of the astrocyte-derived neuritogenic cytokine S100B. Wilcoxon distribution scores for S100B mRNA levels in patients with APOE ϵ4,4 (Group 4) is higher than that in other genotypes. p values: Group 4 vs 1, p = 0.004: Group 4 vs 2, p = 0.001; and Group 4 vs 3, p = 0.004 (A). S100B protein levels for all the patient samples (n = 92), quantified by western blot, showed that APOE ϵ3,3 patients (Group 2, n = 53) had higher levels of S100B than did other APOE genotypes (99.7 ± 5.17 vs 95.7 ± 3.55, 92.7 ± 6.21, 92.9 ± 2.87 arbitrary units; p = 0.001) for groups 1 (n = 13), 3 (n = 19), and 4 (n = 7), respectively (B). Illustration of S100B protein levels; one of eight western blots of different epilepsy samples (n = 92) with standard (Std) purified S100B positive control (middle sample S100B Std) the S100B mono band represents the S100B monomer (~11kD) (C).