TY - JOUR AU - Aboud, Orwa AU - Mrak, Robert E. AU - Boop, Frederick A. AU - Griffin, W. Sue T. PY - 2013 DA - 2013/07/29 TI - Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype JO - Acta Neuropathologica Communications SP - 41 VL - 1 IS - 1 AB - Precocious development of Alzheimer-type neuropathological changes in epilepsy patients, especially in APOE ϵ4,4 carriers is well known, but not the ways in which other APOE allelic combinations influence this outcome. Frozen and paraffin-embedded tissue samples resected from superior temporal lobes of 92 patients undergoing temporal lobectomies as a treatment for medication-resistant temporal lobe epilepsy were used in this study. To determine if epilepsy-related changes reflect those in another neurological condition, analogous tissue samples harvested from 10 autopsy-verified Alzheimer brains, and from 10 neurologically and neuropathologically normal control patients were analyzed using immunofluorescence histochemistry, western immunoblot, and real-time PCR to determine genotype effects on neuronal number and size, neuronal and glial expressions of amyloid β (Aβ) precursor protein (βAPP), Aβ, apolipoprotein E (ApoE), S100B, interleukin-1α and β, and α and β secretases; and on markers of neuronal stress, including DNA/RNA damage and caspase 3 expression. SN - 2051-5960 UR - https://doi.org/10.1186/2051-5960-1-41 DO - 10.1186/2051-5960-1-41 ID - Aboud2013 ER -