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Table 1 Antibodies and conjugates used for immunohistochemistry

From: Calreticulin and other components of endoplasmic reticulum stress in rat and human inflammatory demyelination

Antibody/Conjugate Isotype/Species Supplier Dilution
MOG Mouse mAb Prof R Reynolds, Imperial College London 1/100
BiP/GRP78 Rabbit pAb Abcam (ab32618) 1/100
CRT Mouse mAb Abcam (ab22683) 1/1000
CHOP/GADD153 Mouse mAb Cell signalling (L63F7) 1/200
CHOP/GADD153 Rabbit pAb Santa Cruz biotechnology (sc793) 1/200
p-EIF2a Rabbit mAb Cell signalling (119A11) 1/50
XBP1 Rabbit pAb Santa Cruz biotechnology (sc-7160) 1/100
Iba1 Rabbit pAb Wako (019-19741) 1/1000
GFAP Rabbit pAb Dako (Z0334) 1/1000
GFAP Mouse mAb Sigma (G3893) 1/500
Olig2 Mouse mAb Millipore (MABN50) 1/75
Olig2 Rabbit pAb Chemicon(AB9610) 1/65
NeuN Mouse mAb Millipore (MAB377) 1/1000
CD68 Mouse mAb Calbiochem (CB1014) 1/65
SMI32 Mouse mAb Abcam (ab28029) 1/1000
Biotinylated anti-rabbit Ig Swine pAb Dako (E0353) 1/400
Biotinylated anti-mouse Ig Rabbit pAb Dako (E0354) 1/400
Biotinylated anti-rabbit IgG (H&L) Goat pAb Vector (BA-1000) 1/200
Biotinylated anti-mouse IgG (H&L) Goat pAb Vector (BA-9200) 1/200
Alexafluor 488 anti-rabbit Goat pAb Invitrogen (A11008) 1/1000
Alexafluor 568 anti-rabbit Goat pAb Invitrogen (A11011) 1/1000
Alexafluor 488 anti-mouse Goat pAb Invitrogen (A11059) 1/1000
Alexafluor 568 anti-mouse Goat pAb Invitrogen (A11019) 1/1000
Fluorescein Avidin DCS n/a Vector (A-2011) 1/500
Cy3-conjugated streptavidin n/a Jackson Immuno Research 1/4000