Table 1 Antibodies and conjugates used for immunohistochemistry
Antibody/Conjugate | Isotype/Species | Supplier | Dilution |
|---|---|---|---|
MOG | Mouse mAb | Prof R Reynolds, Imperial College London | 1/100 |
BiP/GRP78 | Rabbit pAb | Abcam (ab32618) | 1/100 |
CRT | Mouse mAb | Abcam (ab22683) | 1/1000 |
CHOP/GADD153 | Mouse mAb | Cell signalling (L63F7) | 1/200 |
CHOP/GADD153 | Rabbit pAb | Santa Cruz biotechnology (sc793) | 1/200 |
p-EIF2a | Rabbit mAb | Cell signalling (119A11) | 1/50 |
XBP1 | Rabbit pAb | Santa Cruz biotechnology (sc-7160) | 1/100 |
Iba1 | Rabbit pAb | Wako (019-19741) | 1/1000 |
GFAP | Rabbit pAb | Dako (Z0334) | 1/1000 |
GFAP | Mouse mAb | Sigma (G3893) | 1/500 |
Olig2 | Mouse mAb | Millipore (MABN50) | 1/75 |
Olig2 | Rabbit pAb | Chemicon(AB9610) | 1/65 |
NeuN | Mouse mAb | Millipore (MAB377) | 1/1000 |
CD68 | Mouse mAb | Calbiochem (CB1014) | 1/65 |
SMI32 | Mouse mAb | Abcam (ab28029) | 1/1000 |
Biotinylated anti-rabbit Ig | Swine pAb | Dako (E0353) | 1/400 |
Biotinylated anti-mouse Ig | Rabbit pAb | Dako (E0354) | 1/400 |
Biotinylated anti-rabbit IgG (H&L) | Goat pAb | Vector (BA-1000) | 1/200 |
Biotinylated anti-mouse IgG (H&L) | Goat pAb | Vector (BA-9200) | 1/200 |
Alexafluor 488 anti-rabbit | Goat pAb | Invitrogen (A11008) | 1/1000 |
Alexafluor 568 anti-rabbit | Goat pAb | Invitrogen (A11011) | 1/1000 |
Alexafluor 488 anti-mouse | Goat pAb | Invitrogen (A11059) | 1/1000 |
Alexafluor 568 anti-mouse | Goat pAb | Invitrogen (A11019) | 1/1000 |
Fluorescein Avidin DCS | n/a | Vector (A-2011) | 1/500 |
Cy3-conjugated streptavidin | n/a | Jackson Immuno Research | 1/4000 |