|
MOG
|
Mouse mAb
|
Prof R Reynolds, Imperial College London
|
1/100
|
|
BiP/GRP78
|
Rabbit pAb
|
Abcam (ab32618)
|
1/100
|
|
CRT
|
Mouse mAb
|
Abcam (ab22683)
|
1/1000
|
|
CHOP/GADD153
|
Mouse mAb
|
Cell signalling (L63F7)
|
1/200
|
|
CHOP/GADD153
|
Rabbit pAb
|
Santa Cruz biotechnology (sc793)
|
1/200
|
|
p-EIF2a
|
Rabbit mAb
|
Cell signalling (119A11)
|
1/50
|
|
XBP1
|
Rabbit pAb
|
Santa Cruz biotechnology (sc-7160)
|
1/100
|
|
Iba1
|
Rabbit pAb
|
Wako (019-19741)
|
1/1000
|
|
GFAP
|
Rabbit pAb
|
Dako (Z0334)
|
1/1000
|
|
GFAP
|
Mouse mAb
|
Sigma (G3893)
|
1/500
|
|
Olig2
|
Mouse mAb
|
Millipore (MABN50)
|
1/75
|
|
Olig2
|
Rabbit pAb
|
Chemicon(AB9610)
|
1/65
|
|
NeuN
|
Mouse mAb
|
Millipore (MAB377)
|
1/1000
|
|
CD68
|
Mouse mAb
|
Calbiochem (CB1014)
|
1/65
|
|
SMI32
|
Mouse mAb
|
Abcam (ab28029)
|
1/1000
|
|
Biotinylated anti-rabbit Ig
|
Swine pAb
|
Dako (E0353)
|
1/400
|
|
Biotinylated anti-mouse Ig
|
Rabbit pAb
|
Dako (E0354)
|
1/400
|
|
Biotinylated anti-rabbit IgG (H&L)
|
Goat pAb
|
Vector (BA-1000)
|
1/200
|
|
Biotinylated anti-mouse IgG (H&L)
|
Goat pAb
|
Vector (BA-9200)
|
1/200
|
|
Alexafluor 488 anti-rabbit
|
Goat pAb
|
Invitrogen (A11008)
|
1/1000
|
|
Alexafluor 568 anti-rabbit
|
Goat pAb
|
Invitrogen (A11011)
|
1/1000
|
|
Alexafluor 488 anti-mouse
|
Goat pAb
|
Invitrogen (A11059)
|
1/1000
|
|
Alexafluor 568 anti-mouse
|
Goat pAb
|
Invitrogen (A11019)
|
1/1000
|
|
Fluorescein Avidin DCS
|
n/a
|
Vector (A-2011)
|
1/500
|
|
Cy3-conjugated streptavidin
|
n/a
|
Jackson Immuno Research
|
1/4000
|
