Figure 5
Upregulation of ER stress molecules in spinal cord grey matter neurons. Semi-quantitative analysis of ER stress molecule expression in neurons of the grey matter showed an upward trend in BiP and CRT expression, when compared to control IFA or saline groups. CHOP, XBP1 and p-eIF2α scores differed significantly from saline controls, while staining of CHOP and p-eIF2α protein also differed significantly from the pattern seen in IFA controls. Histogram data (RH panels) is represented as semi-quantitative immunostaining score / mean arbitrary units (AU)±SEM, n = 5 for all scores. Key: * = p<0.05. Representative images of staining of the grey matter in IFA controls (LH panels) and EAE samples (middle panels), is shown. Scale bars = 50 μm.