Quantification of up-regulation of protein markers of ER stress in spinal cord EAE lesions. Semi-quantitatitive analysis of the spinal cord white matter EAE lesions and lesion edges revealed a trend towards upregulation of BiP, compared to IFA and saline controls (a). Scores for CRT (b), CHOP (c) and p-eIF2α (e) were significantly higher within lesions and the NAWM than those measured in the NAWM of IFA samples. Significant upregulation of CRT, CHOP, XBP1 (d) and p-eIF2α, was found in EAE lesions and NAWM, when compared to saline controls. Key: * = p<0.05; ** = p<0.01; *** = p<0.001. Data is represented as mean arbitrary units (AU) ± SEM.