TMEM106B protein expression in brain tissue from normal controls. TMEM106B protein expression is found throughout all layers of neocortex. Frontal cortex is shown in (a). TMEM106B neuronal and glial staining pattern is similar in both histopathologically affected areas of the brain (b, frontal cortex) and in areas of the brain that are relatively spared of TDP-43 pathology (c, occipital cortex). In the hippocampus, the pyramidal neurons of Ammon’s horn show positive TMEM106B immunoreactivity (d), whereas those of the dentate gyrus do not (e). Lentiform nucleus sections demonstrated very rare neuronal staining (f). There was minimal staining of the Purkinje cells of the cerebellum; rarely, cells demonstrated a cytoplasmic, granular staining, as highlighted in (g). Neurons of the deep cerebellar nuclei demonstrated diffuse cytoplasmic TMEM106B (h). Sections were stained with the anti-TMEM106B polyclonal antibody N2077 . Scale bar represents 50 um.