Figure 3From: Tau pathology induces loss of GABAergic interneurons leading to altered synaptic plasticity and behavioral impairments Loss of GABAergic interneurons in hippocampal area CA1. (a) In situ hybridization for the GABAergic marker, GAD67, showed a significant reduction of GAD67-positive neurons in area CA1 (Scale bar = 100 μm). (b) Fluorescent immunostaining against parvalbumin (PV, Cy3) and somatostatin (SST, Cy2), counterstained with Hoechst (Upper image are tiled images of 200× magnification, lower panel 400× magnification). (c) Tg mice showed significantly reduced numbers of GAD67-positive (WT=9 mice, 69 slices, Tg=8 mice, 60 slices, t 15=2.143, *P=0.0490), SST-positive (WT=6 mice, 61 slices, Tg=7 mice, 61 slices t 11=2.685, *P=0.0212), and PV-positive (WT=7 mice, 61 slices, Tg=7 mice, 61 slices, t 12=3.559, **P=0.0039) neurons in CA1 region in series of brain slices. Data presented as mean + SEM.Back to article page