Loss of GABAergic interneurons in hippocampal area CA1. (a) In situ hybridization for the GABAergic marker, GAD67, showed a significant reduction of GAD67-positive neurons in area CA1 (Scale bar = 100 μm). (b) Fluorescent immunostaining against parvalbumin (PV, Cy3) and somatostatin (SST, Cy2), counterstained with Hoechst (Upper image are tiled images of 200× magnification, lower panel 400× magnification). (c) Tg mice showed significantly reduced numbers of GAD67-positive (WT=9 mice, 69 slices, Tg=8 mice, 60 slices, t
15=2.143, *P=0.0490), SST-positive (WT=6 mice, 61 slices, Tg=7 mice, 61 slices t
11=2.685, *P=0.0212), and PV-positive (WT=7 mice, 61 slices, Tg=7 mice, 61 slices, t
12=3.559, **P=0.0039) neurons in CA1 region in series of brain slices. Data presented as mean + SEM.