Figure 2
Aged Tg mice exhibit altered synaptic plasticity which can be rescued with zolpidem, a GABA A R agonist. (a) Representative traces of field excitatory postsynaptic potentials (fEPSP) in hippocampal slices from aged JNPL3 (BL6) and WT mice that received four trains of high frequency stimulation (HFS) (gray = fEPSP at baseline, black = fEPSP after HFS). (b) Late-phase long-term potentiation (L-LTP) is enhanced in aged JNPL3 (BL6) mice compared to WT controls (ANOVA: WT= 9 mice, 24 slices; Tg=12 mice, 28 slices, F (1,50) =7.841, **p=0.007). (c) Representative traces of fEPSPs in slices from aged JNPL3 (BL6) and WT mice treated with the GABAAR agonist zolpidem and four trains of HFS (gray = fEPSP at baseline, black = fEPSP after HFS). (d) Zolpidem (1 μM for 10’) rescued the enhanced L-LTP in Tg mice while having no effect on L-LTP in WT slices (ANOVA: WT zolpidem=6 mice, 11 slices, Tg zolpidem=7 mice, 17 slices, WT veh= 9 mice, 24 slices, Tg veh=9mice, 28 slices, F (1,78) = 6.841, *p= 0.011. Tukey HSD comparison: Tg zolpidem vs. WT veh: p=0.971). Data presented as mean ± SEM.