Aged JNPL3 (BL6) mice show increased tau pathology in the cortex and hippocampus. (a) Western blot analysis of total tau levels and phospho-tau status in low speed supernatant (LSS) fractions. Total tau levels were significantly increased in Tg mice compared to age-matched WT mice (n=14 per group, t
26=4.616, ***P=0.0002). Early-stage pathological phospho-tau markers, AT180 and CP13, were also significantly increased in cortical tissue from Tg mice (AT180: t
26=3.674, *P=0.0011; CP13: t
26=2.156, *P=0.0405). Immunoblotting with the PHF1 antibody showed a significant shift in the banding pattern from 50 kd to 53 kd in Tg mice when compared to WT controls (t
26=2.527, *P=0.0179). (b) Levels of insoluble sarkosyl pellet tau normalized to LSS total tau are increased in Tg mice compared to controls (t
26=2.215, *P=0.0357). (c) Tg mice showed positive immunostaining with pathological-tau MC1 and PHF1 antibodies. Both antibodies showed staining in the CA1 of JNPL3 (BL6) mice, although to a lesser extent for the PHF1 than MC1 antibody. Inset is higher magnification of the highlighted area. Since brains were fixed with paraformaldehyde without perfusing, blood vessel staining was also present (DG=dentate gyrus, CA1= hippocampal area CA1; Scale bar = 200 μm). Data presented as mean + SEM.