Figure 4

Histological analysis of the lumbar expansion in Dab2wt/wt, Dab2wt/ko and Dab2ko/ko mice subjected to EAE. Representative sections of the lumbar expansion of the spinal cord of a: a wild-type mouse subjected to EAE b: a Dab2 heterozygote mouse subjected to EAE c: a Dab2 knockout mouse subjected to EAE. a/b/c: Orange lines outline immune infiltrates. Scale bar represents 500μm d: Analysis of average number of immune lesions in the spinal cords of Dab2 heterozygous and knockout mice relative to wild-type controls reveals that mice heterozygous for Dab2 and knockout for Dab2 have a similar number of lesions to wild-type mice (p>0.05) e: Analysis of average infiltrate area reveals that the size of immune infiltrates found in the lumbar expansion of Dab2 heterozygous mice and Dab2 knockout mice is significantly smaller than those found in the lumbar expansion of wild-type mice (***p<0.001) Similarly, the average size of immune infiltrates in the spinal cords of Dab2 knockout mice is significantly smaller those found in Dab2 heterozygous mice (*p<0.05). f: Cell densitometry analysis within the core of EAE lesions reveals that there are no significant differences between the proportional composition of various glial cell types within lesions in wild-type and Dab2 heterozygous mice, however, total cell numbers per lesion are significantly less in Dab2 heterozygous and knockout lesions (**p<0.01). Average ± SEM; One-Way ANOVA, Tukey’s post-hoc test; n ≥ 4 mice/genotype for all above analyses.