Figure 1

Dab2 expression is up-regulated in the murine C57B/6 MOG-EAE model relative to health. a: Realtime PCR quantitation of dab2 gene expression in the spinal cords of MOG EAE-immunised mice was expressed relative to healthy controls. Analysis reveals that dab2 gene expression is positively correlated with disease severity (HC: Healthy Control; Spearmann’s rank correlation r2=0.921, p<0.0001). b: Western blot analysis of Dab2 expression in healthy control mice and those subjected to EAE (grade 3.0). 1. Healthy spinal cord 2. EAE spinal cord 3. Healthy forebrain 4. EAE forebrain 5. Healthy hindbrain 6. EAE hindbrain 7. Healthy spleen 8. EAE spleen. Shown is a marked up-regulation of both the p96 and p67 Dab2 isoforms in the EAE spinal cord and hindbrain. c/d/e: Scale bar represents 200μm c: Dab2 staining is undetectable in the healthy spinal cord. d: A region of EAE spinal cord spared from inflammation shows no Dab2 staining. e: A region of EAE spinal cord containing inflammatory infiltrates (DAPI-positive nuclear accumulation) shows Dab2 staining throughout the lesions.